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. 2019 Feb 27;20(5):1027. doi: 10.3390/ijms20051027

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phenethyl isothiocyanate (PEITC) suppresses HeLa cancer stem cells (hCSCs) proliferation. (a) Representative photomicrographs showing attenuation of hCSCs-sphere formation (day 7) by PEITC in a concentration dependent manner (400-μm scale). (b) Bar graphs showing concentration-dependent effects of PEITC on the viability of hCSCs after 24 h treatment. The dotted line represents the baseline cell viability for dimethyl sulfoxide (DMSO), which served as a vehicle control to determine statistical significance. (c) Bar graphs showing the percentage of early apoptotic cells obtained from Propidium Iodide and Annexin V assay. All data represent means ± SEM, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.