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. 2019 Feb 28;20(5):1047. doi: 10.3390/ijms20051047

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of exogenous Ca²⁺ on 1-aminocyclopropane-l-carboxylic acid synthase (ACS) activity (A), ACC Oxidase (ACO) activity (B), 1-aminocyclopropane-1-carboxylic acid (ACC) production (C), and ethylene production (D) in cucumber explants under salt stress at different times. The primary roots were removed from hypocotyl of five-day-old seedlings. Explants were incubated for five days with distilled water as control, meanwhile explants were incubated with 13 mM NaCl after being treated with distilled water or 10 μM CaCl₂, 500 μM lanthanum chloride (LaCl₃), 100 μM methylene glycol-bis (2-aminoethylether) -N,N,N′,N′-tetraacetic acid (EGTA), 500 μM LaCl₃ + 10 μM CaCl₂, and 100 μM EGTA + 10 μM CaCl₂, respectively. Values are expressed as means ± SE (n = 3), Bars with different lowercase letters were significantly different by Duncan’s multiple range test (P < 0.05).

Effect of exogenous Ca²⁺ on 1-aminocyclopropane-l-carboxylic acid synthase (ACS) activity (A), ACC Oxidase (ACO) activity (B), 1-aminocyclopropane-1-carboxylic acid (ACC) production (C), and ethylene production (D) in cucumber explants under salt stress at different times. The primary roots were removed from hypocotyl of five-day-old seedlings. Explants were incubated for five days with distilled water as control, meanwhile explants were incubated with 13 mM NaCl after being treated with distilled water or 10 μM CaCl₂, 500 μM lanthanum chloride (LaCl₃), 100 μM methylene glycol-bis (2-aminoethylether) -N,N,N′,N′-tetraacetic acid (EGTA), 500 μM LaCl₃ + 10 μM CaCl₂, and 100 μM EGTA + 10 μM CaCl₂, respectively. Values are expressed as means ± SE (n = 3), Bars with different lowercase letters were significantly different by Duncan’s multiple range test (P < 0.05).