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. 2019 Feb 28;20(5):1047. doi: 10.3390/ijms20051047

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of Ca²⁺ on the Expression Levels of ACS gene CsACS1 (A), CsACS2 (B), CsACS3 (C), and ACO gene CsACO1 (D), CsACO2 (E) in cucumber explant under salt stress at 48 h. The primary roots were removed from hypocotyl of 5-day-old seedlings. Explants were incubated for five days with distilled water as control, meanwhile the explants were incubated with 13 mM NaCl after being treated with distilled water or 10 μM CaCl₂, 500 μM LaCl₃, 100 μM EGTA, 500 μM LaCl₃ + 10 μM CaCl₂, and 100 μM EGTA + 10 μM CaCl₂, respectively. The values (means ± SE) are the average of three independent experiments. Bars with different lowercase letters were significantly different by Duncan’s multiple range test (P < 0.05).

Effects of Ca²⁺ on the Expression Levels of ACS gene CsACS1 (A), CsACS2 (B), CsACS3 (C), and ACO gene CsACO1 (D), CsACO2 (E) in cucumber explant under salt stress at 48 h. The primary roots were removed from hypocotyl of 5-day-old seedlings. Explants were incubated for five days with distilled water as control, meanwhile the explants were incubated with 13 mM NaCl after being treated with distilled water or 10 μM CaCl₂, 500 μM LaCl₃, 100 μM EGTA, 500 μM LaCl₃ + 10 μM CaCl₂, and 100 μM EGTA + 10 μM CaCl₂, respectively. The values (means ± SE) are the average of three independent experiments. Bars with different lowercase letters were significantly different by Duncan’s multiple range test (P < 0.05).