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. 2019 Mar 12;20(5):1244. doi: 10.3390/ijms20051244

Figure 4.

Figure 4

Rh2 suppressed paracrine effects of SASP in breast cell lines. (A) Senescent MDA-MB-231 and MCF-10A were exposed to Rh2 for 48 h, after which caspase 3/7 activity was quantified. The dotted line indicates fluorescence level in nontreated senescent cells. (B) MDA-MB-231 and MCF-10A cells were cultured with indicated CM for 24 h. The cell viability was assayed by WST-1 method. (C) Fold changes in mRNA levels of IL-8, IL-6, CXCL1, and MCP-1. The dotted line indicates expression level in control cells, set at 1 for each gene. N = 3. A.U., arbitrary units. (D) ELISA assay of IL-6 and IL-8. * indicates p < 0.05 versus Non-treated con. ** indicates p < 0.01 versus Non-treated con; *** indicates p < 0.001 versus Nontreated con. # indicates p < 0.05 versus CM group. ## indicates p < 0.01 versus CM group. ### indicates p < 0.001 versus CM alone group.