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. 2019 Mar 5;24(5):913. doi: 10.3390/molecules24050913

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AAEE, AAEE-Pe, and AAEE-Ea induced apoptosis in BEL-7404 and H22 cells. Cells were treated with different concentrations of AAEE, AAEE-Pe, and AAEE-Ea for 24 h. After staining with Annexin V and PI, BEL-7404 (A) and H22 (B) cells were analyzed by flow cytometry. (C) Proteins were isolated, and the levels of Bax and Bcl-2 were analyzed by Western blot. * p < 0.05; *** p < 0.001 compared to Untreated.