Skip to main content
. 2019 Mar 5;24(5):913. doi: 10.3390/molecules24050913

Figure 3.

Figure 3

AAEE, AAEE-Pe, and AAEE-Ea induced cell cycle arrest in BEL-7404 and H22 cells. BEL-7404 and H22 cells were treated with different concentrations of AAEE, AAEE-Pe, and AAEE-Ea for 24 h. (A) BEL-7404 and H22 cells were stained with Hoechst 33258 and observed by inverted fluorescence microscope. (B) DNA contents in BEL-7404 cells were analyzed by flow cytometry and are shown in the upper panels. The summaries of the cell cycle distributions in BEL-7404 and H22 cells are shown in the middle and bottom panels, respectively. (C) Expression of Cyclin B1 was analyzed by Western blot. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to Untreated.