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Nitrogenase activity of P. graminis RSA19 (WT), Δorf1 (deletion mutant), Δorf1/orf1 (complementation strain) and WT/orf1 (overexpression strain) under iron limitation. These strains were grown anaerobically in a normal nitrogen-limited medium (Control) and nitrogen-limited medium without ferric citrate (-Fe). The nitrogenase activities of these strains were assayed by C2H4 reduction method and expressed as nmol C2H4/mg protein/hr.
