Table 2.
Primers used in this study.
| Primer Name | Sequence | Location/Target |
|---|---|---|
| For qRT-PCR | ||
| 16SF | TTTGTCGTCAGCCTCGTGTTCGTG | qRT-PCR for control (16S rDNA) |
| 16SR | ATCCCCACCTTCCTCCGGTTTG | |
| RBF | GCATCCGCCATGTCACTATCACC * | qRT-PCR for nifB |
| RBR | CGCACACCTTCGTCGAACACC | |
| RHF | GACTCCACACGCCTGATTCTGAAC * | qRT-PCR for nifH |
| RHR | CCGCCGCACTCTACGTTGATG * | |
| RDF | CACTGCCACCGCTCCATGAAC * | qRT-PCR for nifD |
| RDR | CACGCAGGCTCTCATAAGTCTTGG * | |
| RKF | CAGTCATCTCAGCCGCCACTTC * | qRT-PCR for nifK |
| RKR | TCCAAGCCGTCGATCAGATTGTTC * | |
| REF | CGGTCATCCCAGTGAACAGC * | qRT-PCR for nifE |
| RER | CCCGCACTGTTCATCAGCTT * | |
| RNF | ACACCGCTGATTGCAGGAATGG * | qRT-PCR for nifN |
| RNR | TGCCGTGCGAATTGCTGATCC * | |
| RXF | GCGGTGAGCTGCTAGAACTGC * | qRT-PCR for nifX |
| RXR | GGCTGCCGAACGGAACCTTAAC * | |
| ROF | GACTTCAACTGCGCCGTATCTCC * | qRT-PCR for orf1 |
| ROR | TCCGCTCTTCTCTTCCATGACTCC * | |
| RAF | GTTGAAGGAAGCGACGGTGATGG * | qRT-PCR for hesA |
| RAR | GACCAGAATCAGCTTGCCGACAC | |
| RVF | AGGAGGATATTGCGGCGATTGC | qRT-PCR for nifV |
| RVR | CCGAGACAGGAATAGACACATGGC * | |
| For construction of orf1 deletion and overexpression strains | ||
| orf1UF | CGGCCACGATGCGTCCGGCGTAGAGGATCCGCTGGGGACCCCCTATAAG | Upstream of orf1 |
| orf1UR | TAAATTTGCAGCTTGGCTTCCTCCCCTTCCC | |
| orf1DF | GGGGAGGAAGCCAAGCTGCAAATTTAGGATCGG | Downstream of orf1 |
| orf1DR | TCATGGCGACCACACCCGTCCTGTGGATCCGCTTGCTCAACTCCGCATTC | |
| nifPF | TTATAACAGGAATTCCCGGGGATCCTGCTGCTTCCTCCTCATTTG | Promoter region of nif operon |
| nifPR | ACGATTCCATTTCCCACCTCCTAAAAGTAAC | |
| orf1F | GAGGTGGGAAATGGAATCGTCGGCTAAC | orf1 ORF |
| orf1R | ATGGAAAAACGCTTTGCCCAAGCTTATCCTTCAAGGCTGAAC | |
* Primers also used in the RT-PCR.