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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Steps within a single round of APE-Gen. From left to right: anchor alignment, peptide backbone sampling, and energy minimization. After a round is complete, the highest quality conformation as determined by the scoring function is used to initialize the next round.