Table 2.
Primer sequences used for real-time RT-PCR.
| Species | Primer | Forward (5′–3′) | Reverse (5′–3′) | bp |
|---|---|---|---|---|
| Human | SOX2 | GCCCTGCAGTACAACTCCAT | GACTTGACCACCGAACCCAT | 128 |
| NANOG | AAGGCCTCAGCACCTACCTA | TGCACCAGGTCTGAGTGTTC | 181 | |
| OCT4 | GATGTGGTCCGAGTGTGGTT | AGCCTGGGGTACCAAAATGG | 160 | |
| PPARγ | CTAAAGAGCCTGCGAAAG | TGTCTGTCTCCGTCTTCTTG | 331 | |
| LPL | TCAACTGGATGGAGGAG | GGGGCTTCTGCATACTCAAA | 169 | |
| ALP | CAACAGGGTAGATTTCTCTTGG | GGTCAGATCCAGAATGTTCC | 135 | |
| BGLAP | GGCAGCGAGGTAGTGAAGAG | CAGCAGAGCGACACCCTAGAC | 195 | |
| VEGF | CCCACTGAGGAGTCCAACAT | TTTCTTGCGCTTTCGTTTTT | 186 | |
| bFGF | CGACCCTCACATCAAGCTACA | CGTTTCAGTGCCACATACCAA | 219 | |
| HGF | ATCAAATGTCAGCCCTGGAG | TCGATAACTCTCCCCATTGC | 207 | |
| ANGPT-1 | CATTCTTCGCTGCCATTCTG | GCACATTGCCCATGTTGAATC | 103 | |
| ANGPT-2 | ACTGTGTCCTCTTCCACCAC | GGATGTTTAGGGTCTTGCTTT | 132 | |
| PDGF | GCAAGACCAGGACGGTCATTT | GGCACTTGACACTGCTCGT | 135 | |
| SDF-1α | TCAGCCTGAGCTACAGATGC | CTTTAGCTTCGGGTCAATGC | 161 | |
| GAPDH | GGACTCATGACCACAGTCCATGCC | TCAGGGATGACCTTGCCCACAG | 152 | |
| Mouse | Il-1β | GGTCAAAGGTTTGGAAGCAG | TGTGAAATGCCACCTTTTGA | 94 |
| Mmp9 | GCCCTGGAACTCACACGACA | TTGGAAACTCACACGCCAGAAG | 85 | |
| Cldn3 | AAGCCGAATGGACAAAGAA | CTGGCAAGTAGCTGCAGTG | 72 | |
| β-actin | TCCCTGGAGAAGAGCTATGA | CGATAAAGGAAGGCTGGAA | 100 |