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. 2019 Feb 28;20(5):1055. doi: 10.3390/ijms20051055

Figure 7.

Figure 7

Overexpression analysis of the PsMYB114L gene in apple calli. (A) Colors observed in transgenic apple calli and the WT. (B) Results of positive PCR detection in transgenic apple calli. (C) Anthocyanin extraction solutions for transgenic apple calli and the WT. (D) Total anthocyanin content in transgenic apple calli and the WT. (E) Expression analysis of anthocyanin biosynthesis-related genes in transgenic apple calli and the WT. WT, Wild-type ‘Orin’ apple calli; PsMYB114L-1 and PsMYB114L-2, two transgenic lines of the PsMYB114L gene. Different lowercase letters indicate significant differences at p < 0.05.