Table 2.
GC-MS linkage analysis of barley β-glucan (BBG) and its acid hydrolyzed derivatives.
Samples | Peak Area (%) a | Relative Molar Ratio c T-Glcp:1,3-Glcp:1,4-Glcp | Relative Molar Ratio c T-Glcp:1,3-Glcp:1,4-Glcp | ||
---|---|---|---|---|---|
T-Glcp b | 1,3-Glcp | 1,4-Glcp | |||
BBG | 3.99 ± 1.00 | 14.25 ± 0.15 | 81.76 ± 0.85 | 1:3.57:20.49 | 0.28:1:5.74 |
BBG_0.05 | 3.76 ± 0.94 | 14.15 ± 0.14 | 82.09 ± 0.80 | 1:3.77:21.84 | 0.27:1:5.80 |
BBG_0.1 | 13.22 ± 2.99 | 13.11 ± 0.45 | 73.68 ± 2.54 | 1:0.99:5.58 | 1.01:1:5.62 |
BBG_0.2 | 22.46 ± 4.55 | 9.94 ± 0.58 | 67.60 ± 3.97 | 1:0.44:3.01 | 2.26:1:6.80 |
BBG_0.2(2) | 32.28 ± 5.73 | 9.24 ± 0.78 | 58.48 ± 4.94 | 1:0.29:1.81 | 3.49:1:6.33 |
a: calculated from the peak area in the GC-MS total ion chromatograms. b: Glcp, glucopyranose. c: molar ratio was calculated from the ratio of the (peak area)/(MW) of individual PMAAs.