Table 2.

ANOVA for the quadratic model adjusted to the extraction of total phenolic compounds.

	Source	Coefficient	Sum of Squares	Degrees of Freedom	Mean Square	F-Value	p-Value
	Model		2940.04	27	108.89	2.31	0.0179
Methanol	X1	−1.15	31.97	1	31.97	0.6787	0.4175
Temperature	X2	−1.01	24.50	1	24.50	0.5202	0.4772
Amplitude	X3	0.0166	0.0066	1	0.0066	0.0001	0.9906
Cycle	X4	−4.17	418.01	1	418.01	8.88	0.0062
pH	X5	−0.8371	16.82	1	16.82	0.3571	0.5553
Ratio	X6	0.5498	7.26	1	7.26	0.1541	0.6979
Methanol × Temperature	X1X2	6.75	364.53	1	364.53	7.74	0.0099
Methanol × Amplitude	X1X3	1.01	8.14	1	8.14	0.1729	0.6809
Methanol × Cycle	X1X4	−2.00	63.93	1	63.93	1.36	0.2545
Methanol × pH	X1X5	4.38	153.56	1	153.56	3.26	0.0826
Methanol × Ratio	X1X6	−0.7961	5.07	1	5.07	0.1077	0.7454
Temperature × Amplitude	X2X3	−0.7882	4.97	1	4.97	0.1055	0.7479
Temperature × Cycle	X2X4	0.6373	3.25	1	3.25	0.0690	0.7949
Temperature × pH	X2X5	2.87	132.23	1	132.23	2.81	0.1058
Temperature × Ratio	X2X6	−2.36	44.72	1	44.72	0.9495	0.3388
Amplitude × Cycle	X3X4	−4.26	144.96	1	144.96	3.08	0.0911
Amplitude × pH	X3X5	−1.14	10.47	1	10.47	0.2222	0.6413
Amplitude × Ratio	X3X6	1.87	56.01	1	56.01	1.19	0.2855
Cycle × pH	X4X5	−2.64	55.80	1	55.80	1.18	0.2863
Cycle × Ratio	X4X6	6.71	359.66	1	359.66	7.64	0.0104
pH × Ratio	X5X6	1.68	22.66	1	22.66	0.4812	0.4940
Methanol × Methanol	X12	−8.06	668.48	1	668.48	14.19	0.0009
Temperature × Temperature	X22	−0.2020	0.4197	1	0.4197	0.0089	0.9255
Amplitude × Amplitude	X32	1.79	32.81	1	32.81	0.6967	0.4115
Cycle × Cycle	X42	1.87	36.12	1	36.12	0.7669	0.3892
pH × pH	X52	2.96	90.01	1	90.01	1.91	0.1786
Ratio × Ratio	X62	−2.20	49.57	1	49.57	1.05	0.3144
Residual		45.85	1224.48	26	47.10
Lack of Fit			1075.70	21	51.22	1.72	0.2858
Pure Error			148.78	5	29.76
Total			4164.52	53