Table 3.

ANOVA for the quadratic model adjusted to the extraction of total anthocyanins.

	Source	Coefficient	Sum of Squares	Degrees of Squares	Mean Square	F-Value	p-Value
	Model		2514.45	27	93.13	1.47	0.1631
Methanol	X1	4.80	553.02	1	553.02	8.75	0.0065
Temperature	X2	−2.05	100.49	1	100.49	1.59	0.2185
Amplitude	X3	−1.88	85.15	1	85.15	1.35	0.2563
Cycle	X4	−2.47	146.43	1	146.43	2.32	0.1400
pH	X5	1.13	30.92	1	30.92	0.4892	0.4905
Ratio	X6	−0.1120	0.3008	1	0.3008	0.0048	0.9455
Methanol × Temperature	X1X2	−5.59	250.28	1	250.28	3.96	0.0572
Methanol × Amplitude	X1X3	−4.68	174.94	1	174.94	2.77	0.1082
Methanol × Cycle	X1X4	−2.18	76.24	1	76.24	1.21	0.2821
Methanol × pH	X1X5	6.51	338.73	1	338.73	5.36	0.0288
Methanol × Ratio	X1X6	0.0864	0.0598	1	0.0598	0.0009	0.9757
Temperature × Amplitude	X2X3	−0.1454	0.1691	1	0.1691	0.0027	0.9591
Temperature × Cycle	X2X4	−1.16	10.77	1	10.77	0.1704	0.6831
Temperature × pH	X2X5	−3.14	157.76	1	157.76	2.50	0.1262
Temperature × Ratio	X2X6	0.8116	5.27	1	5.27	0.0834	0.7751
Amplitude × Cycle	X3X4	3.73	111.16	1	111.16	1.76	0.1963
Amplitude × pH	X3X5	−1.63	21.28	1	21.28	0.3368	0.5667
Amplitude × Ratio	X3X6	−1.04	17.31	1	17.31	0.2739	0.6052
Cycle × pH	X4X5	−0.7232	4.18	1	4.18	0.0662	0.7990
Cycle × Ratio	X4X6	0.2305	0.4249	1	0.4249	0.0067	0.9353
pH × Ratio	X5X6	−0.9561	7.31	1	7.31	0.1157	0.7365
Methanol × Methanol	X12	−1.95	39.12	1	39.12	0.6191	0.4385
Temperature × Temperature	X22	2.23	50.98	1	50.98	0.8068	0.3773
Amplitude × Amplitude	X32	2.89	86.14	1	86.14	1.36	0.2536
Cycle × Cycle	X42	−1.17	14.04	1	14.04	0.2221	0.6414
pH × pH	X52	2.03	42.24	1	42.24	0.6683	0.4211
Ratio × Ratio	X62	−1.64	27.72	1	27.72	0.4386	0.5136
Residual		23.34	1643.02	26	63.19
Lack of Fit			1469.28	21	69.97	2.01	0.2248
Pure Error			173.75	5	34.75
Total			4157.47	53