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. 2019 Feb 27;20(5):1042. doi: 10.3390/ijms20051042

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SK-OV-3 cells are overexpressing CD44. (A) Western blot analysis of human ovary cancer derived cells probed with anti-hCD44 MAb and human beta-actin antibody. (B) Relative intensity of the bands of CD44 to beta-actin in Western blot was densitometrically analyzed by ImageJ. (C) Relative gene expression levels of CD44 to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were analyzed by RT-qPCR. The data presented as the mean ± SD from three independent experiments. The statistical significance in mean values of more than two groups was determined using one-way analysis of variance (ANOVA) and Dunnet multiple comparison test using CD44 expression of SK-OV-3 cells as control; (****) p < 0.001.