Fig. 3.

Detection of JAK2^V617F mutation. CD14⁺ cells were isolated using CD14⁺ isolation kits (Miltenyi Biotec, Germany), then cells were analyzed at Genoptix Company (Carlsbad, CA) for the JAK2^V617F mutation analysis. The real time PCR amplification of wildtype or JAK2^V617F mutation is shown as cycle number (X-axis) vs. fluorescence intensity (Y-axis). The darker purple curve furthest of the upper right is the WT probe and the lower right curve is the mutant probe. The Ct for the wild type probe was 25.2032 and the Ct for the V617F probe was 28.1533. The% mutated in this patient was 33.58%.