Fig. 1.

PCTS Culture device. a: Photograph illustrating device assembly. Two polyether ether ketone (PEEK) plastic plates, each with a medial recess to house a sintered pyrex disk, affix to sandwich a silicone gasket with a central cavity in which the precision cut tissue slice (PCTS) is contained. A 70 μM porous nylon membrane separates the tissue from the sintered disk (not shown). b: Photograph of an assembled PCTS culture device with external dimensions. c: Schematic of PCTS culture device. Component parts numerically labelled: 1, PEEK plastic plates; 2, glass sintered pyrex disk; 3, central silicone gasket; 4, tumour tissue sample