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. 2019 Mar 22;19:259. doi: 10.1186/s12885-019-5465-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Percentage Ki-67 positivity in fresh (+ 0 h) and post-culture (+ 96 h) thyroid tumour tissue. Mean Ki-67 positivity was 1.52 ± 0.52% and 1.3 ± 0.34% in fresh and post-culture tissue, respectively; no significant difference was observed (n = 8; p = 0.32 [paired t-test]). Inset: Representative photomicrographs of Ki67, BrdU and TTF1 positive brown nuclei and counterstained with haematoxylin (× 400). BrdU staining is only shown in cultured tissue due to the requirement of perfusion with the molecule in order to elicit its incorporation and subsequent detection; 12.09 ± 4.38% cells stained positive for BrdU incorporation (n = 3)