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. 2019 Mar 21;19:253. doi: 10.1186/s12885-019-5455-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Heat map and hit validation using a pool of siRNAs in PANC-1 and HPNE cells. Heat map showing cell confluence (measured with the IncuCyte Live Cell Analysis System) after transfection of siRNA into a PANC-1 cells and b HPNE cells. PSMA6 is shown in red text and indicated by an arrow. The cell confluence is color coded: blue represents low confluence and red represents high confluence, as measured by live-cell imaging over 66 h. Samples were normalized to controls treated with 1 μM staurosporine (as a positive control for cell death) and lipid reagent alone