Fig. 5.

miR-423-5p may play a role in the proangiogenic activity of hADSC-derived exosomes. a RT-qPCR was used to determine the expression profile of the top 30 miRNAs in the exosomes and hADSCs. Delta CT was used to show the expression level of miRNAs relative to the internal reference RNU6: the larger the value, the lower the expression. b The basal expression level of the top30 miRNAs in HUVECs was determined using RT-qPCR. Delta CT was used to compare the expression level of miRNAs to the internal reference RNU6. (c) The changes of miR-423-5p, miR-320a and miR-143-3p after uptake of the exosomes by the HUVECs for 24 h was determined using RT-qPCR. Cells treated with PBS served as the control. d The changes in Sufu after HUVEC uptake of the exosomes for 48 h was determined using RT-qPCR. Cells treated with PBS served as the control. e–f Changes in the expression of Sufu in HUVECs 48 h after transfection with the miR-423-5p mimic were determined using RT-qPCR (e) and Western blotting (f). g–h Representative images of scratch assays recorded at 0 h and 12 h after transfection of the HUVECs with the miR-423-5p mimic. i–j Representative images of vessel-like formation assay recorded at 0 h and 12 h after plated the HUVECs transfected with the miR-423-5p mimic