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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Pharmacol Res. 2017 Dec 5;129:515–525. doi: 10.1016/j.phrs.2017.11.036

Figure 1.

Figure 1.

Adenosine A2A and histamine H3 receptors co-immunoprecipitate in transfected HEK-293 cells. A. Co-immunoprecipitation of the hemagglutinin-tagged H3R (3xHA-H3R) with the A2AR in protein extracts from HEK-293 cells. The ~45 kDa band corresponds to the 3xHA-H3R, and was not observed with the negative control. Input represents 30% of the total protein. An antibody against the D2 receptor (α-D2R) was used as a negative control. The blot is representative of 3 independent experiments. The whole blot is shown in Supplementary Figure 5.