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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Pharmacol Res. 2017 Dec 5;129:515–525. doi: 10.1016/j.phrs.2017.11.036

Table 3.

Analysis of binding assays in rat striatal membranes

pKi Bmax (%)
[3H]-NAMH
Synaptosomes
   RAMH 9.09 ± 0.22 100.0 ± 0.3
   RAMH + CGS 10 nM 8.99 ± 0.02ns 110.0 ± 4.0ns
   RAMH + CGS 100 nM 9.08 ± 0.06ns 103.0 ± 4.1ns

[3H]CGS-21680
Whole striatum
   CGS 8.06 ± 0.15 100.0 ± 14
   CGS + RAMH 8.11 ± 0.04ns 88.0 ± 6.0ns
Synaptosomes
   CGS 8.10 ± 0.04 100.0 ± 9.0
   CGS + RAMH 7.70 ± 0.04a 88.1 ± 10.0ns

Data are means ± SEM from 3–5 experiments. For [3H]-NAMH binding assays the statistical analysis was performed with one-way Anova and Dunnett´s post hoc test. For [3H]-CGS-21680 binding, values in the presence of RAMH were compared with the corresponding control with Student’s t test.

a

P < 0.001, ns, not significant. CGS, CGS-21680. RAMH (R-α-methylhistamine) was tested at 100 nM in all the experiments.