. Author manuscript; available in PMC: 2019 Mar 22.

Published in final edited form as: Pharmacol Res. 2017 Dec 5;129:515–525. doi: 10.1016/j.phrs.2017.11.036

Table 3.

Analysis of binding assays in rat striatal membranes

	pKi	Bmax (%)
[³H]-NAMH
Synaptosomes
RAMH	9.09 ± 0.22	100.0 ± 0.3
RAMH + CGS 10 nM	8.99 ± 0.02^ns	110.0 ± 4.0^ns
RAMH + CGS 100 nM	9.08 ± 0.06^ns	103.0 ± 4.1^ns

[³H]CGS-21680
Whole striatum
CGS	8.06 ± 0.15	100.0 ± 14
CGS + RAMH	8.11 ± 0.04^ns	88.0 ± 6.0^ns
Synaptosomes
CGS	8.10 ± 0.04	100.0 ± 9.0
CGS + RAMH	7.70 ± 0.04^a	88.1 ± 10.0^ns

Data are means ± SEM from 3–5 experiments. For [³H]-NAMH binding assays the statistical analysis was performed with one-way Anova and Dunnett´s post hoc test. For [³H]-CGS-21680 binding, values in the presence of RAMH were compared with the corresponding control with Student’s t test.

P < 0.001, ns, not significant. CGS, CGS-21680. RAMH (R-α-methylhistamine) was tested at 100 nM in all the experiments.