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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Nat Protoc. 2019 Jan;14(1):248–282. doi: 10.1038/s41596-018-0093-7
Reagent Volume (μl) per reaction Final concentration
2× KAPA HiFi HotStart ReadyMix 5 1×
10 μM Forward primer (RP1; Table 1) 1.25 1.25 μM
10 μM Reverse primer (RPIn; Table 1) 1.25 1.25 μM