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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Sci Signal. 2017 Dec 12;10(509):eaan2392. doi: 10.1126/scisignal.aan2392

Fig. 4. RNA-seq reveals impairment of the amplification phase of the IFN response to RIG-I stimulation in older monocytes.

Fig. 4.

Human monocytes from younger (n=2) and older (n=3) healthy donor blood were transfected with a RIG-I–specific 5’-ppp 14-bp dsRNA ligand. After 6 hours, RNA was isolated from these cells and was used for exploratory RNA sequencing. (A) Volcano plot of differentially regulated genes. Normalized values of transcripts encoding both (B) type I/III IFNs and (C) IRFs were compiled into heat maps using Microsoft Excel in order to highlight trends in differential induction of these genes.