Figure 2.

Ectopic Ski expression increases MMP2 gelatinase activity in primary cardiac myofibroblasts. First passage (P1) primary cardiac MFB were grown to 70% confluency, and infected with either AdLacZ or AdSki at 50 and 100 MOI for 48 h. Untreated control lysates were serum‐deprived, but not infected with either virus. (A and C) Representative gelatin zymographs of total cell lysates for intracellular MMP‐2 in MFB treated with 50 MOI adenovirus (Fig. 2A) or 100 MOI adenovirus (Fig. 2C). Native human MMP‐2 protein (5 μg) was used as a positive control in Figure 2A. (B and D) Histograms showing data obtained in Figures 2A and C, respectively. The level of intracellular MMP‐2 activity was significantly increased in response to Ski overexpression, compared to Ad‐LacZ and untreated controls. Data are representative of the means ± SEM with n = 3. **P < 0.01, compared to uninfected and Ad‐LacZ infected controls.