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. 2019 Mar 18;12:641–653. doi: 10.2147/IDR.S191892

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© 2019 Xu et al. This work is published and licensed by Dove Medical Press Limited

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The S1-PFGE and Southern hybridization analysis of 15 Klebsiella pneumoniae strains co-harboring bla_KPC-2 and rmpA2.

Notes: (A) S1 nuclease digestion of genomic DNA of K. pneumoniae strains was followed by PFGE. Plasmid bands are shown as linearized fragment on the gel. (B) Southern blot hybridization of the marker gene (rmpA2) of the virulence plasmid. (C) Southern blot hybridization of bla_KPC-2. Lane M, reference standard strain Salmonella serotype Braenderup H9812 restricted with Xbal.

Abbreviations: M, marker; S1-PFGE, S1 nuclease pulsed-field gel electrophoresis.