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. 2019 Mar 19;5(3):e01376. doi: 10.1016/j.heliyon.2019.e01376

Fig. 5.

Fig. 5

Antinociceptive effects of 5′-chloro-2′-hydroxychalcone (6) and 5′-methyl-2′-hydroxy-3′-nitrochalcone (12) in mice. A) Effect of the i.p. injection of chalcone 6 and 12 (10 mg/kg) on the acetic acid induced writhing (n = 6–14 mice per group). B) Effect of the i.p. injection of chalcone 6 and 12 (30 mg/kg) on the hot plate tests (n = 6–12 mice per group). Effect of acute administration of 5′-methyl-2′-hydroxy-3′-nitrochalcone (12) in C) the acetic acid induced writhing (0.3, 3, 10 and 30 mg/kg) (n = 6–16 mice per group); D) the hot plate (10 and 30 mg/kg) (n = 8–12 mice per group) and E) locomotor activity tests (10 and 30 mg/kg) in mice (n = 7–8 mice per group). Results are expressed as mean ± S.E.M. of the number of writhes, the reaction time of mice in the hot plate test and spontaneous locomotor activity counts in comparison to control animals (injected with vehicle, VEH). Statistical analysis was performed by one-way ANOVA followed by Dunnett's test. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group.