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. 2017 Sep 23;6(11):1997–2008. doi: 10.1002/sctm.17-0091

Figure 6.

Figure 6

Stromal cell‐derived factor‐1 (SDF‐1) mediates ERC‐based therapy in increasing the percentage of both regulatory T cell (Tregs) and regulatory B cell (Bregs) in allograft recipients. Splenocytes were collected from B6 recipients in each group at postoperative day 8. For the Tregs, the percentage of CD25+Foxp3+ cells in CD4+ gating population was determined by fluorescence‐activated cell sorting analysis. (A): Dot plots of CD4+CD25+Foxp3+ T cells. (B): Percentage of CD4+CD25+Foxp3+ T cells. For the Bregs, the percentage of CD83+ (C), CD1d+CD5+ (D), or IL‐10+ (E) cells in CD19+ gating population was measured. *ERCs indicated inhibition the function of SDF‐1 by AMD3100. Statistical analysis was done by one‐way analysis of variance followed by the least significant difference test, n = 6. Abbreviations: ERC, endometrial regenerative cell; RAPA, rapamycin.