Figure 1.

Formulation of Solubilized Amnion Membrane Hydrogel. (A): Process of generating solubilized amnion membrane from human placental tissue. Amnion membrane is manually dissected from the chorion membrane and washed with sterile saline to remove blood. The membrane is cut into small pieces, washed in saline and sterile water, and lyophilized/freezer milled. This amnion is then digested in pepsin/HCl and the supernatant is collected. (B): A diagram demonstrating the reaction by which the HA‐based Extracel hydrogel is formed by thiol‐ene photopolymerization chemistry. Thiolated HA and gelatin are cross‐linked with PEGDA in the presence of the photoinitiator 4‐(2‐hydroxyethoxy)phenyl‐(2‐hydroxy‐2‐propyl)ketone using irradiation with UV light. Abbreviations: HA, hyaluronic acid; PEGDA, polyethylene glycol diacrylate.