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. 2017 Sep 23;6(11):2020–2032. doi: 10.1002/sctm.17-0053

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© 2017 The Authors stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Quantitative Analysis of Wound Healing. (A): Percentage (%) wound remaining was calculated by dividing the area of the remaining wound by the original wound size (C/A × 100). HA‐SAM groups had significant acceleration of wound closure times resulting in wound closure 3–4 days before other groups. (B): Wound re‐epithelialization was calculated by measuring newly re‐epithelized skin, taking into consideration remaining wound area and contraction ([B‐C]/B × 100). HA‐SAM groups had significantly greater wound re‐epithelialization at all time‐points compared with other groups. (C): Contraction was measured based on original wound size and the area of re‐epithelized skin ([A‐B]/A × 100). HA only and HA‐SAM groups had significantly less contraction compared with untreated animals. (D): Wound aspect ratio was determined to describe observed changes in the shape and direction of wound contraction between groups (length:width). HA only and HA‐SAM groups displayed symmetrical contraction, with aspect ratios close to 1, while other groups showed asymmetrical contraction with aspect ratios closer to 4.