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. 2019 Mar 18;12:2063–2070. doi: 10.2147/OTT.S195022

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© 2019 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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MMP16 is a potential target of miR-328-3p in osteosarcoma cells.

Notes: (A) The binding sites of miR-328-3p to MMP16 mRNA and the relative luciferase activity of miR-328-3p overexpressed U2OS cells cotransfected with the constructs containing the WT or the MUT 3′-UTR of MMP16. (B) Western blot analysis of the relative expression levels of MMP16 protein in U2OS cells with the enforced expression of miR-328-3p. Each assay was conducted in triplicate and repeated thrice. The data show the mean of triplicate repeats ± SD.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR, microRNA; MMP16, matrix metalloprotease 16; MUT, mutant; NC-mimics, negative control mimics; WT, wild type.