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. 2019 Mar 18;12:2063–2070. doi: 10.2147/OTT.S195022

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© 2019 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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MMP16 overexpression partially reverses the miR-328-3p-inhibited cell proliferation and migration of osteosarcoma cell.

Notes: (A) The expression levels of MMP16 protein in U2OS cells cotransfected with MMP16-ex and miR-328-3p-mimic, or with NC-ex and miR-328-3p-mimic. (B) Absorbance of U2OS cells cotransfected with MMP16-ex and miR-328-3p-mimic, or with NC-ex and miR-328-3p-mimic at five consecutive time points (0, 24, 48, 72, and 96 hours) detected by CCK-8 assay. (C and D) The representative images and the number of migrating U2OS cells cotransfected with MMP16-ex and miR-328-3p-mimic, or with NC-ex and miR-328-3p-mimic detected by Wound healing assay. Each assay was conducted in triplicate and repeated thrice. The data show the mean of triplicate repeats ± SD.

Abbreviations: CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR, microRNA; MMP16, matrix metalloprotease 16; MMP16-ex, MMP16 expression plasmid; NC, negative control; NC-ex, negative control expression plasmid.