Fig. 5.

Effects of incubation with PDI-poor or PDI-rich plasma on endothelial cell expression of several genes covering different biological processes. EC were incubated with 5% PDI-poor plasma, PDI-rich plasma or FBS for 24 h and the expression levels of pre-specified 84 genes were analyzed using RT² Profile PCR array. A. Heat map of relative expressions of transcripts. B. Transcript levels are shown as Log₂ fold-changes for genes which were simultaneously upregulated with PDI-poor and downregulated with PDI-rich plasma. C. Transcript levels are shown as Log₂ fold-changes for genes with distinct behavior in response to incubation with PDI-poor or PDI-rich plasma. All expression values were normalized for the expression of β-2-microglobulin. All data represent mean values from 3 independent experiments.