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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Nature. 2018 Jul 18;559(7715):637–641. doi: 10.1038/s41586-018-0350-5

Fig. 2 |. TET2 is a substrate of AMPK, which specifically phosphorylates TET2 at S99.

Fig. 2 |

a, Conserved AMPK recognition motif of several key AMPK target enzymes, including TET2. b, Co-immunoprecipitation showing that Flag–TET2 interacts with the activated form of AMPK (AMPKpT172). c, Flowchart of the in vitro kinase assay with different detection approaches. d, LC–MS/MS profiling of the in vitro AMPK kinase assay showed that TET2S99 is specifically phosphorylated in the presence of active AMPK. e, Immunoblot validation of TET2pS99 phosphorylation using a TET2pS99-specific antibody. f, Determination of TET2 phosphorylation at S99 via 32P-autoradiograph (SLF is a naturally occurring mutant that probably disrupts the AMPK binding site). Data represent three biologically independent repeats in b, e, f.