Table 1.
The most enriched aptamer sequences obtained by NGS
| Aptamer | Sequences 5′-GGCUCGAGG-(N25)-GGAAGCUUACGGUACCUAGC-3′ | Population (%) | Enrichment (fold) | |||
|---|---|---|---|---|---|---|
|
|
|
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| S16 | S3 | S1 | S3/S1 | S16/S3 | ||
| APT1 | AACGUACAGAGGGUGGAGAGUGGGU | 3.0×101 | 1.2×101 | 3.6×10−2 | 338 | 2.5 |
| APT2 | ACGUAACAGAGGGAGGCGAGUGGGU | 2.6×101 | 1.3×101 | 3.1×10−2 | 399 | 2.1 |
| APT3 | ACCGAAGUCGAGGGGGACGUGAGGG | 7.9×10−1 | 4.3×10−1 | 1.0×10−3 | 419 | 1.8 |
| APT4 | ACCUAAGUGGGAAGGUGAGCGGGUG | 2.8×10−1 | 1.0×10−1 | 6.9×10−4 | 149 | 2.7 |
| APT5 | AACGUACAGAGGGCGGAGAGUGGGU | 2.5×10−1 | 1.2×10−1 | 6.9×10−5 | 1676 | 2.2 |
| APT6 | AAAUGCAAGGAGGUCGGAGGUCGGA | 2.3×10−1 | 1.4×10−1 | 5.5×10−4 | 255 | 1.6 |
| APT7 | ACCGACGACGAGGGGGACGUGAGGG | 2.0×10−1 | 7.3×10−2 | 1.1×10−3 | 66 | 2.8 |
| APT8 | AUAAGCAUGGGGGUCGAGAGGGGAC | 2.0×10−1 | 1.9×10−1 | 1.2×10−2 | 15 | 1.1 |
| APT9 | AACGUACAGAGGGAGGCGAGUGGGU | 1.8×10−1 | 2.8×10−1 | 1.0×10−3 | 274 | 0.6 |
| APT10 | AACGUACAGAGGGUGGAGAGUGGGU | 1.8×10−1 | 7.0×10−2 | 1.0×10−3 | 67 | 2.6 |
Sequence information and percentage of ten aptamers were addressed from libraries S1, S3, and S16. Ratios of frequency of S3/S1 and S16/S3 were calculated, showing large increases in their populations at early rounds of SELEX.