Fig. 1.

Study design of the nonalcoholic fatty liver disease (NAFLD) model. Fig. 1 represents the overall flow of the study. A total of 24 mice were classified into 2 groups, normal fat diet (NFD, green-bordered) and high fat diet (HFD, yellow-bordered). Each group consisted of 6 wild type mice (WTNF or WTHF, light blue-colored) and 6 catalase knockout mice (CKONF or CKOHF, light orange-colored). To observe the effect of HFD (liver fat accumulation) and CKO (oxidative stress and inflammation), statistical analysis and pathway analysis were employed. The comparison between WTNF vs. CKOHF was excluded after preanalysis.