Fig. 2.

Effects of EC on METH-induced apoptosis in HT22 hippocampal neuronal cells. Cells were pretreated with EC for 1 h, and incubated with 5 mM METH for 24 h. Cells were stained with PI and Annexin V, and then evaluated by flow cytometry (left). Percentage of apoptotic cells was detected by flow cytometry (right). Results are shown as mean ± SEM from three independent experiments. ^*p<0.01 when compared with untreated control cells. ^#p<0.01 when compared with METH-treated cells.