Fig. 3.

Effects of EC on METH-induced ROS accumulation and reduction of MMP in HT22 hippocampal neuronal cells. (A) Cells were pretreated with EC for 1 h, and incubated with 1 mM METH for 24 h. Cells were then incubated with 5 μM DCF-DA for 15 min. The intracellular ROS level was determined using a fluorescence microscope. (B) Cells were pretreated with EC for 1 h, and treated with 5 mM METH for 24 h. Cells were stained with TMRM and the fluorescence was measured by flow cytometry (left). Percentage of MMP was calculated and plotted in the graph (right). ^*p<0.01 when compared with untreated control cells. ^#p<0.01 when compared with METH-treated cells.