Fig. 4.

Effects of EC on METH-induced protein expression in HT22 hippocampal neuronal cells. Cells were pretreated with 20 μM EC for 1 h, treated with 5 mM METH at various time intervals (A, C), or pretreated with 10 μM or 20 μM EC for 1 h and then treated with 5 mM METH for 24 h (B, D). Cell lysates were resolved by SDS-PAGE and then analyzed by Western blot using antibodies against p38, p-p38, ERK, p-ERK, CHOP, DR4 and β-actin. The results shown were obtained from at least three independent experiments.