Fig. 4.

Administration of 6-shogaol or 6-paradol suppresses microglial activation in spinal cords of EAE mice. Effects of 6-shogaol (Shog) and 6-paradol (Para) on microglial activation were examined by Iba1 immunolabeling. (A) Representative images of Iba1 immunoreactivity in the lumbar spinal cord of each group. (B) Quantification of the number of Iba1-immunopositive cells. Scale bar, 50 μm. n=4 per group. ^***p<0.001 and ^###p<0.001 vs. naïve and the vehicle-treated group, respectively, by Newman-Keuls multiple range test.