Table 3.

One-to-one Langmuir fitting parameters to anti-biotin reactions with surface biotin-bovine serum albumin and biotin-polyvinyl alcohol conjugates. The loading was specified by the molar ratio of biotin to bovine serum albumin (40×, 20×, 10×, and 5×) or the percentage of biotin to polyvinyl alcohol (4%, 2%, and 1%).[28]

Loading	40×	2×	10×	5×	4%	2%	1%
On-rate (nMs)−1×105	1.954	2.489	4.939	4.797	3.333	5.807	9.553
Off-rate (s)−1×106	4.665	5.909	7.659	6.111	13.93	10.26	7.093
Equilibrium dissociation constant (nM)	2.387	2.374	1.551	1.274	4.179	1.767	0.742