Table 4.

One-to-two Langmuir fitting parameters to anti-biotin reactions with surface biotin-bovine serum albumin and biotin-polyvinyl alcohol conjugates. The loading was specified by the molar ratio of biotin to bovine serum albumin (40×, 20×, 10×, and 5×) or the percentage of biotin to polyvinyl alcohol (4%, 2%, and 1%).[28]

loading	40×	20×	10×	5×	4%	2%	1%
1 On-rate (nMs)−1×105	4.87	2.10	1.53	4.12	2.25	3.67	2.46
1 Off-rate (s)−1×104	3.19	3.69	4.91	5.25	8.03	8.14	10.4
1 Equilibrium dissociation constant (nM)	6.55	17.57	32.09	12.74	35.69	22.18	40.81
Site one occupation ratio	0.3	0.35	0.41	0.34	0.16	0.27	0.61
					0.53	0.61	0.6
					0.61	0.58	0.35
					0.55	0.39	0.3
2 On-rate (nMs)−1×105	0.98	2.12	6.96	3.04	2.04	3.06	6.31
2 Off-rate (s)−1	<9×10−7	<2×10−6	<3.6×10-6	<4.9×10-6	<1.9×10-6	<1×10−6	<7×10−7
2 Equilibrium dissociation constant (nM)	<0.092	<0.094	<0.052	<0.161	<0.093	<0.033	<0.011