Table 5.

One-to-one Langmuir fitting parameters to anti-2, 4-dinitrophenol reactions with surface biotin-2, 4-dinitrophenol and biotin-2, 4-dinitrophenol conjugates. The loading was specified by the molar ratio of biotin to bovine serum albumin (40×, 20×, 10×, and 5×) or the percentage of biotin to polyvinyl alcohol (4%, 2%, and 1%).[28]

Loading	40×	20×	10×	5×	1.5%	0.75%
On-rate (nMs)−1×105	0.998	0.793	1.256	2.042	0.438	2.288
Off-rate (s)−1×106	2.862	5.481	9.871	19.1	6.839	12.95
Equilibrium dissociation constant (nM)	0.287	0.691	0.786	0.935	1.56	0.566