Table 6.

One-to-two Langmuir fitting parameters to anti-2, 4-dinitrophenol reactions with surface biotin-2, 4-dinitrophenol and biotin-2, 4-dinitrophenol conjugates. The loading was specified by the molar ratio of biotin to bovine serum albumin (40×, 20×, 10×, and 5×) or the percentage of biotin to polyvinyl alcohol (4%, 2%, and 1%).[28]

Loading	40×	20×	10×	5×	1.5%	0.75%
1 On-rate (nMs)−1×106	1.76	0.19	1.23	4.19	2.34	0.46
1 Off-rate (s)−1×105	1.14	1.64	8.45	7.87	116	96.5
1 Equilibrium dissociation constant (nM)	6.48	86.32	68.70	18.78	495.7	2099
Site one occupation ratio	0.58	0.89	0.53	0.42	0.05	0.15
					0.05	0.26
					0.06	0.03
					0.05	0.18
2 On-rate (nMs)−1×105	2.46	2.75	2.01	4.40	0.39	2.21
2 Off-rate (s)−1	<1.4×10−6	<1.1×10−6	<1.7×10−6	<1.7×10−6	<3×10−7	<1.2×10−6
2 Equilibrium dissociation constant (nM)	<0.057	<0.040	<0.085	<0.039	<0.077	<0.054