Table 2.
Genes and primers used for relative quantification by qRT-PCR in the liver of lambs
| Target genea | Primer sequences (5′–3′) | Product length (bp) | Annealing temperature, °C | Accession number |
|---|---|---|---|---|
| GAPDH | F: ACCACTTTGGCATCGTGGAG R: GGGCCATCCACAGTCTTCTG |
75 | 61 | NM_002046 |
| β actin | F: CTTGATGTCACGGACGATTT R: CACGGCATTGTCACCAACT |
380 | 61 | NM_001101.4 |
| ATP7A | F: TGATGATGAGCTGTGCGGTT R: CATTGCTACCCGTTTCCCCT |
241 | 61 | NM_000052.6 |
| MIa | F: CACTGACCACACTTCCCTACA R: AGGACTCTGGAGGGTTCACAA |
226 | 63 | NM_177389.3 |
| Cu/Zn SOD | F: GACTTGGGCAGAGGTGGAAA R: CAGGGAATGTTTACGGGGCA |
100 | 61 | NM_000454.4 |
| GPx1 | F: CCTGGTCGTACTCGGCTTC R: CCTTCTCGCCATTCACCTC |
154 | 61 | NM_000581.3 |
| GPX4 | F: GGGAGTAATGCGGAGATCAA R: CATACCGCTTCACCACACAG |
210 | 61 | NM_001039847.2 |
a GAPDH glyceraldehyde 3-phosphate dehydrogenase, ACTB β-actin, MIa metallothionein-Ia, SOD Cu/Zn superoxide dismutase, GPx1 glutathione peroxidase 1, GPx4 glutathione peroxidase 4