TABLE 1.
Summary of genome elements, methyltransferase genes, and their motifs identified in Deinococcus wulumuqiensis 479
| Genetic element | GenBank accession no. | Genome size (bp) | Genome coverage (×) | Methylase (RM system) namea | Recognition motifb | Methylation, RM type |
|---|---|---|---|---|---|---|
| Chromosome | CP031158 | 2,658,929 | 423.73 | RM.DrdVIIIc | ARGAGC | 6mA, IIG |
| RM.DrdVc | CATGNAC | 6mA, IIG | ||||
| DrdIX | GNGAYNNNNNCTC | 6mA, I | ||||
| Plasmids | ||||||
| pDrdI | CP031163 | 668,667 | 536.84 | M.DrdIc | GACNNNNNNGTC | 6mA, II |
| M.DrdORFCP | Not active | 6mA, I | ||||
| M.DrdVII | YCGCGR | 5mC II | ||||
| pDrdA | CP031159 | 311,711 | 511.45 | |||
| pDrdB | CP031160 | 70,951 | 678.58 | |||
| pDrdIV | CP031162 | 31,268 | 544.7 | RM.DrdIVc | TACGAC | 6mA, IIG |
| pDrdII | CP031161 | 21,536 | 544.9 | RM.DrdIIc | GAACCA | 6mA, IIG |
| pDrdVI | CP031164 | 15,512 | 243.93 | M1-2.DrdVIc | GCAGCC | 6mA, IIG |
a
RM, restriction modification.
b
Modified bases are in bold and bases opposite to them are bold and underlined.
c
Methyltransferase (MTase) genes cloned and expressed in E. coli strain ER2796 (11). DrdIII activity has been previously reported (1). Recent analysis of this strain did not reveal any traces of DrdIII activity nor the presence of a CGATCG modified motif. The strain may have lost a plasmid encoding the DrdIII gene, or the enzyme called DrdIII may have come from a contaminant.