Table 2.
Recovery of PEth 16:0/18:1 using different elution solvents for 96‐well SLE
| PEth 16:0/18:1 concentration | n | Elution solvent(s) | Recoverya | |||||
|---|---|---|---|---|---|---|---|---|
| (nmol/L) | ng/mL | MTBE (%) | 2‐propanol (%) | Ethylacetate (%) | Heptane (%) | Recovery (%) | RSD (%) | |
| 100 | 70 | 4 | 100 | 0 | 0 | 0 | 3 | 10 |
| 100 | 70 | 4 | 90 | 10 | 0 | 0 | 24 | 10 |
| 100 | 70 | 4 | 80 | 20 | 0 | 0 | 49 | 2 |
| 100 | 70 | 4 | 70 | 30 | 0 | 0 | 58 | 8 |
| 100 | 70 | 4 | 60 | 40 | 0 | 0 | 62 | 7 |
| 100 | 70 | 4 | 0 | 0 | 100 | 0 | 13 | 10 |
| 100 | 70 | 4 | 0 | 0 | 90 | 10 | 10 | 14 |
| 100 | 70 | 4 | 0 | 0 | 80 | 20 | 5 | 13 |
| 100 | 70 | 4 | 0 | 0 | 70 | 30 | 3 | 12 |
| 100 | 70 | 4 | 0 | 0 | 60 | 40 | 2 | 39 |
a
Recovery for each set was determined as: (Average calculated concentration of samples added PEth before extraction/Average calculated concentration of samples added PEth 16:0/18:1 after extraction) × 100. Internal standard was added after extraction in all samples.