Table 4.

Discordant results in the three real‐time PCR assays

No.	Specimen	Anyplex	AdvanSure	PowerChek	Culture	AFB stain	Diagnosis
1	Br. asp	MTB	‐	MTB	No growth	‐	TB pleurisy
2	Br. asp	MTB	‐	‐	MTB	1+	Pulmonary TB
3	Br. asp	MTB	‐	MTB	No growth	‐	COPD, pneumonia
4	Br. asp	MTB	‐	MTB	MTB	‐	Pulmonary TB
5	Sputum	MTB	‐	‐	MTB	‐	Pulmonary TB
6	Sputum	NTM	‐	NTM	No growth	‐	COPD
7	Sputum	NTM	‐	‐	No growth	‐	Pneumonia
8	Sputum	NTM	‐	‐	No growth	‐	Pneumonia

AFB, acid‐fast bacilli; Br. asp, bronchial aspirate; COPD, chronic obstructive pulmonary disease; MTB, Mycobacterium tuberculosis; NTM, nontuberculous mycobacteria; TB, tuberculosis.