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. 2019 Mar 22;14(3):e0214373. doi: 10.1371/journal.pone.0214373

Fig 2. O2 consumption rate of Holothuria scabra at different temperatures, obtained from four warm and cold experimental replications.

Fig 2

Points represent means ± s.d. of eight biological replicates (n = 8). Upper red dotted line indicate temperature-induced maximal metabolic rate (TMMR), at the critical warm temperature (WTcrit; 38°C), lower red dotted line marks the start of the temperature-induced standard metabolic rate (TSMR), at critical cold temperature (CTcrit; 22°C). The difference between TMMR and TSMR define the temperature-induced aerobic scope (TAS) for H. scabra (black brace). Beyond CTcrit and WTcrit (22–38°C) progressively negative aerobic performance caused by growing dependency on energy reserves and declining aerobic capacity, respectively, indicate homeostatic disruption. Overexpression of Hsp70 occurs only at the warm temperature maximum (41°C).